Figure 8.

Proliferation of ASMCs induced by TNF-α. (A) DNA synthesis in ASMCs stimulated with TNF-α (1 ng/ml) for 24 h was assessed by methyl-[³H]-thymidine incorporation. ^*P<0.05 vs. ASMCs without lentivirus vector treatment; ^#P<0.05 vs. ASMCs from normal rats without TNF-α stimulation or lentivirus vector treatment; ^##P<0.01 vs. ASMCs from normal rats with TNF-α stimulation and without lentivirus vector treatment; ^NSP<0.05 vs. ASMCs from normal rats with TNF-α stimulation and lentivirus vector treatment. (B) Cell number of ASMCs stimulated with TNF-α (1 ng/ml) for 24 h was detected by hemocytometer. ^*P<0.05 vs. ASMCs without lentivirus vector treatment; ^##P<0.01 vs. ASMCs from normal rats with TNF-α stimulation and without lentivirus vector treatment; ^NSP<0.05 vs. ASMCs from normal rats with TNF-α stimulation and lentivirus vector treatment. Data are presented as the mean ± standard error of the mean of eight independent experiments. TRPM7, transient receptor potential cation channel subfamily M member 7; shRNA, short hairpin RNA; TNF-α, tumor necrosis factor-α; ASMCs, airway smooth muscle cells.