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. 2016 Jun;22(6):883–895. doi: 10.1261/rna.053272.115

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Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — TH mRNA is present in axonal polysomes. qRT-PCR analysis of polysomal RNA from axons or cell body of SCG cell cultures (7 DIV). The histogram shows the percentage of TH mRNA that co-sediments with polysomes in polysomes isolated in the presence of EDTA relative to polysomes isolated in the absence of EDTA Treatment of polysomes with EDTA significantly decreased the levels of TH RNA bound to polysomes, demonstrating the purity of the isolated polysome fraction. CB, polysome-bound TH RNA from cell bodies; CB + EDTA, polysome-bound TH RNA from cell bodies + EDTA treatment. Data are mean ± SEM from three independent experiments. Student's t-test, (***) P ≤ 0.001.