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. 2016 Jun;22(6):883–895. doi: 10.1261/rna.053272.115

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Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Local translation of TH mRNA facilitates axonal dopamine synthesis. (A) Intra-axonal TH and dopamine levels were measured using immunocytochemistry in axons lipofected with TH mRNA (upper panel) or sham-transfected (lower panel). Increased dopamine levels are detected in axons locally overexpressing TH. (B,C) Fluorescence intensity as a measure of TH (B) and dopamine (C) levels was quantified using ImageJ, and fluorescence levels are indicated as relative fluorescence intensity. Data are mean ± SEM from the measurement of 20–24 axons from three independent experiments. Student's t-test, (***) P ≤ 0.0001. (D) Western-blot analysis of axonal and cell body protein lysates from SCG axons transfected with TH mRNA or transfection reagent. β-actin was used as a loading control. Blots of axonal and cell body TH proteins showed significant overexpression of TH protein levels in axons transfected with TH mRNA, whereas TH levels in parental cell soma from transfected axons remained at basal levels.