Fig. 3. Reintegration occurs in multiple epithelia.
A) Inscuteable expression causes spindle misorientation in the optic lobe neuroepithelium. The two daughter cells are tracked in (C). This image is representative of the Inscuteable-induced spindle misorientation in this tissue quantified in Egger et al14.
B) Expression of Inscuteable reorients mitotic spindles in the early embryonic ectoderm. Spindles are perpendicular to the plane of the epithelium. These spindles are among the 34 quantified in Supplemental Figure 3B.
C) Both products of a misoriented division are maintained in the neuroepithelial layer. Pseudo-colouring indicates the two division products. By the 12’ minute timepoint, the basal daughter has reattached to the apical surface. After this point it moves orthogonally to the plane of focus and is no longer observed. This is one of two complete reintegrations imaged in this tissue.
D) Reintegration of two basally misplaced cells following misoriented divisions in the embryonic ectoderm. Arrows point to correctly positioned daughter cells. The basally mispositioned daughters are marked by asterisks. These reintegrations, representing two of the three tracked, were exceptional; other misplaced daughter cells could not be tracked due to the rapid morphogenetic movements of the tissue and the limited depth resolution.
Scale bars in this figure represent 10μM.