Extended Data Table 2. Phosphorylation sites of Apc1 and Apc3 subunits of in vitro phosphorylated APC/C identified by mass spectrometry.

Protein	Phospho-sites	APC/CWT	APC/CWT	APC/CΔApc3-loop	APC/CWT	APC/CWT	APC/CWT	References
	Kinases used	No treatment	Cdk2-cyclinA3	Cdk2-cyclinA3-Cks2 + Plkl	Cdk2-cyclinA3-Cks2	Plkl	Cdk2-cyclinA3-Cks2 + Plkl
Apc1	LVG S60LQE							3
	QEVT65IHE							3(interphase)
	PPGS202PRE							1,2,3
	LFGS233SRV	N.D.					N.D.	2
	LKFS286EQG							1,3
	QGGT291PQN							1,2,3
	NVAT297SSS
	VATS298SSL
	ATSS299SLT
	RSLS309KGD			N.D.				3(interphase)
	KGDS313PVT							3
	SPVT316SPF							3
	PVTS317PFQ							3
	HSRS341PSI							1,2,3
	RSPS3431SN							1,3
	AALS351RAH							3
	RAHS355PAL							1,2,3
	GVHS362FSG							1,2,3
	HSFS364GVQ							1,3
	FNIS372SHN							3
	NISS373HNQ							1,3
	HNQS377PKR							1,2,3
	ISHS386PNS						N.D.	3(interphase)
	SPNS389NSN						N.D.	3
	SNGS394FLA
	WTET4161TN
	VLYT501GVV
	PAPS518LTM	N.D.	N.D.	N.D.				3
	PSLT520MSN	N.D.	N.D.					1(interphase),3
	LTMS522NTM	N.D.	N.D.					3(interphase)
	MSNT524MPR	N.D.	N.D.					3(interphase)
	RPST530PLD							1(interphase),2,3
	DGVS536TPK
	GVST537PKP							1,3
	KPLS542KLL		N.D.					2
	LLGS547LDE							1,3
	VLLS555PVP							1,2,3
	LRDS563SKL							3
	RDSS564KLH	N.D.						2,3
	LHDS569LYN							1(interphase),3
	EDCT576FQQ
	QLGT582YIH
	LELS600NGS			N.D.				2
	FEGS686LSP							3
	GSLS688PVI							1,2,3
	ARPS699ETG							3
	PSET701GSD							3
	ETGS703DDD							3(interphase)
	LCLS731PSE							l,3(interphase),2
	NRFS916FRH							3
	FRHS920TSV
	RHST921SVS
	HSTS922VSS
	VLSS1001DVP
	KHKS1347PSY
	KSPS1349YQI
Apc3	LPNS183CTT							3
	NSCT185TQV
	SCTT186QVP
	PNHS192LSH
	HSLS194HRQ
	QPET200VLT
	TVLT203ETP							2
	LTET205PQD							1,2,3
	PQDT2091El							1
	NLES219SNS	N.D.						3(interphase)
	LESS220NSK	N.D.						2,3
	SSNS222KYS							3
	SKYS225LNT
	SLNT228DSS
	NTDS230SVS
	TSDS231VSY
	SSVS233YID
	YIDS237AVI
	AVIS241PDT							3
	SPDT244VPL							1,3
	GTGT251SII
	TGTS252SIL							3
	SILS255KQV
	KPKT264GRS	N.D.			N.D.			2
	TGRS267LLG				N.D.
	AALS276PLT							3
	SPLT279PSF							3
	LTPS281FGI
	PLET289PSP
	ETPS291PGD						N.D.	1
	QNYT302NTP							1,3
	YTNT304PPV							1,3
	DVPS312TGA							1,3
	VPST313GAP							1,3
	IGQT327GTK	N.D.	N.D.			N.D.		3
	QTGT329KSV
	GTKS331VFS	N.D.	N.D.			N.D.
	SVFS334QSG	N.D.				N.D.		2
	FSQS336GNS	N.D.				N.D.		2
	REVT343PIL							1
	LAQT349QSS
	QTQS351SGP
	TQSS352GPQ
	GPQT356STT
	PQTS357TTP
	QTST358TPQ
	TSTT359PQV
	QVLS364PTI							1
	LSPT3661TS							1,2
	PTIT368SPP
	TITS369PPN							1,2,3
	RLFT383SDS							2
	LFTS384DSS							2
	TSDS386STT							2
	SDSS387TTK							2
	DSST388TKE
	SSTT389KEN							2
	GGIT419QPN
	INDS426LEI							1,2
	LEIT430KLD							1
	KLDS434S11							1
	LDSS435llS							1,2
	SIIS438EGK							2
	GKIS443TIT
KIST4441TP							2,3
STIT446PQI							1,2
MNFS761WAM
IMGT800DES
TDES803QES
SQES806SMT
QESS807MTD
SSMT809DAD
ADDT814QLH
AAES821DEF

The pink shading shows the presence of phosphorylation sites and the white indicates its absence.

Following references were used for comparison:

^1.Kraft et al. EMBO J 2003

^2.Steen et al. PNAS 2008

^3.Hegemann et al. Sci Signal 2011

The phosphorylation sites within the Apel Al segment are highlighted in light purple shadows. Phosphorylation sites of the remaining APC/C subunits are summarized in Extended Data Table 3.