Skip to main content
. Author manuscript; available in PMC: 2016 May 24.
Published in final edited form as: Mol Microbiol. 2016 Feb 28;100(2):345–361. doi: 10.1111/mmi.13321

Figure 6. PccA interacts with SenC in vivo.

Figure 6

The indicated strains were incubated with para-formaldehyde (PFA) (+) or the reaction buffer only (−). After membrane preparation, cells were solubilized with DDM, and PccAHis was purified via metal affinity chromatography. Purified protein was then separated on SDS-PAGE, transferred to a PVDF membrane and decorated with antibodies against SenC (upper panel) or PccA (lower panel). GK32 carries a ccoNO deletion and lacks cbb3-Cox. In the ΔccoGHIS strain, the cbb3-Cox assembly factors CcoGHIS are absent.