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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Traffic. 2015 Nov 16;16(12):1318–1329. doi: 10.1111/tra.12331

Figure 2. LUCID vector design.

Figure 2

(A) Representative LUCID vector showing low copy plasmid for co-expression of FLuc-tagged cargo under native promoter (SNA3-FLuc) and soluble RLuc driven by the constitutive PGK1 promoter (PGK1pr::RLuc). ori, bacterial origin of replication. URA3, yeast selectable marker. CEN/ARSH4, yeast centrosomal sequence and origin of replication. AmpR, bacterial ampicillin resistance gene. Parallel LoxP sequences or AatII cut sites enable removal of the CEN/ARSH4 locus, converting the plasmid from replicating to integrating. (B) Demonstration of LUCID assay using either replicating or chromosome-integrated plasmids. Bars each represent the mean of 3 biological replicates.