Figure 2.

Validating multiplex beads to assess plasma immunoglobulin A (IgA) titers after oral administration of rotavirus and polio vaccines. A, Correlation between plasma IgA titers determined by the rotavirus beads in our multiplex assay compared with those determined by the gold standard rotavirus-specific enzyme-linked immunosorbent assay (ELISA). B, Correlation of neutralization titers for poliovirus serotype 3 (OPV3) determined by standard microneutralization assays and plasma titers of total anti-OPV3 IgA by multiplex assay. C, Correlation of plasma titers of anti–poliovirus serotype 1 (OPV1) IgA and anti-OPV3 IgA determined by multiplex assays. In all panels, each circle represents 1 subject. Spearman coefficient (r) and P values represent results of a nonparametric Spearman correlation test.