Table 1. Details and sequence of primers used for qPCR assays.
| Gene | GenBank a | Sequence (5’-3’) b | Size c | Tm d | Efficiency e |
|---|---|---|---|---|---|
| Hamster β-actin | AF046210 | (F) TCTACAACGAGCTGCG | 357 | 88.33 | 1.802±0.04 |
| (R) CAATTTCCCTCTCGGC | |||||
| Murine β-actin | NM_007393 | (F) AAGAGAAGCTGTGCTATGTT | 251 | 86.25 | 1.660±0.09 |
| (R) GTTGGCATAGAGGTCTTTACG | |||||
| Hamster MIP-1α/CCL3 | AY819019.1 | (F) CTCCTGCTGCTTCTTCTA | 210 | 84.97 | 1.856±0.06 |
| (R) TGGGTTCCTCACTGACTC | |||||
| Murine MIP-1α/CCL3 | NM_011337.2 | (F) TCAGACACCAGAAGGATAC | 159 | 84.64 | 1.780±0.03 |
| (R) CTGAGAAGACTTGGTTGC | |||||
| Hamster IP-10/CXCL10 | AY007988.1 | (F) CTCTACTAAGAGCTGGTCC | 150 | 83.38 | 1.900±0.06 |
| (R) CTAACACACTTTAAGGTGGG | |||||
| Murine IP-10/CXCL10 | NM_021274.1 | (F) CTTAACCACCATCTTCCCAA | 152 | 76.62 | 1.581±0.03 |
| (R) GATGACACAAGTTCTTCCA | |||||
| Hamster GAPDH | DQ403055 | (F) CCGAGTATGTTGTGGAGTCTA | 170 | 85.67 | 1.938±0.04 |
| (R) GCTGACAATCTTGAGGGA | |||||
| Murine GAPDH | NM_008084 | (F) TCATCCCAGAGCTGAACG | 213 | 86.39 | 1.853±0.02 |
| (R) GGGAGTTGCTGTTGAAGTC | |||||
| Hamster IL-1β | AB028497.1 | (F) ATCTTCTGTGACTCCTGG | 156 | 85.29 | 1.852±0.04 |
| (R) GGTTTATGTTCTGTCCGT | |||||
| Murine IL-1β | NM_008361 | (F) GTGTGGATCCCAAGCAATAC | 161 | 83.35 | 1.660±0.09 |
| (R) GTTGTTTCCCAGGAAGACAG | |||||
| Hamster IL-10 | AF046210 | (F) TGGACAACATACTACTCACTG | 308 | 85.50 | 1.871±0.05 |
| (R) GATGTCAAATTCATTCATGGC | |||||
| Murine IL-10 | NM_010548 | (F) ATTCCCTGGGTGAGAAG | 259 | 83.45 | 1.891±0.04 |
| (R) CTCTGTCTAGGTCCTGG | |||||
| Hamster TNF-α | AF046215 | (F) AACGGCATGTCTCTCAA | 278 | 88.05 | 1.849±0.03 |
| (R) AGTCGGTCACCTTTCT | |||||
| Murine TNF-α | NM_013693 | (F) CAACGGCATGGATCTCA | 325 | 87.80 | 1.832±0.04 |
| (R) GGACTCCGCAAAGTCT | |||||
| Hamster iNOS | NM_001281644 | (F) CCATTCTACTACTATCAGGTCG | 274 | 88.4 | 1.844±0.04 |
| (R) TGCCCTTGTACTGGTTCAT | |||||
| Murine iNOS | NM_010927.4 | (F) CCTCATGCCATTGAGTTC | 349 | 88.5 | 1.920±0.04 |
| (R) AGTCATGTTTGCCGTC | |||||
| Hamster TGF-β1 | AF046214 | (F) ACGGAGAAGAACTGCT | 245 | 89.7 | 1.859 ±0.04 |
| (R) ACGTAGTACACGATGGG | |||||
| Murine TGF-β1 | NM_011577.2 | (F) ACCGCAACAACGCCATCTAT | 200 | 86.01 | 1.788±0.03 |
| (R) GTA ACG CCA GGA ATT GTT GC | |||||
| Leptopira lfb1 f | LA0322 | (F) CATTCATGTTTCGAATCATTTCAAA f | 331 | 83.40 | 1.735±0.03 |
| (R) GGCCCAAGTTCCTTCTAAAAG f | |||||
| Leptospira lipL32 g | - | (F) AAG CAT TAC CGC TTG TGG TG | 242 | - | 1.788±0.03 |
| (R) GAACTCCCATTTCAGCGATT | |||||
| (P) AAAGCCAG GACAAGCGCCG |
a Accession Number of mRNA sequence in GenBank (NCBI) used for primer design.
b (F), (R) and (P) indicate forward and reverse primer and probe sequences, respectively.
c PCR product size in base pairs.
d PCR product melting temperature (Tm) in °C.
e Efficiency for PCR was determined by elaboration of standard curves as described in Materials and Methods.