Fig 6. Effect of Ani9 on ANO2 activity.
A-C) YFP Fluorescence measurement showing inhibition of mouse ANO2 activity by Ani9, T16Ainh-A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16Ainh-A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16Ainh-A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).