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. 2016 May 24;11(5):e0155937. doi: 10.1371/journal.pone.0155937

Fig 1. Mapping of new victr mutant alleles isolated via facile DFPM-mediated root growth arrest.

Fig 1

A) Overview and amino acid changes (marked with stars) in three newly isolated EMS mutants in the VICTR locus. B) EMS point mutants in VICTR (Col-0), victr-6, victr-7 and victr-8, showed insensitivity to DFPM similarly to T-DNA mutant victr-1 whereas the wild-type Col-0 control was fully arrested in root growth assays. The black horizontal bars mark the root tip position when plants were exposed to 10 μM DFPM. The vertical grey bars separate the indicated genotypes grown on the same plate for clarity. C) Quantitative analyses of root growth assays shown in B). With 10 μM DFPM all three EMS victr point mutants and victr-1 showed a similar DFPM insensitivity compared to the their growth in 0 μM DFPM. Wild type Col-0 showed a strong inhibition in root growth upon DFPM treatment. Error bars represent SEM (n = 7–18 seedlings for 10 μM DFPM, n = 3–17 for 0 μM DFPM).