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. 2016 May 24;11(5):e0155937. doi: 10.1371/journal.pone.0155937

Fig 3. DFPM treated-wild type (Col-0) seedlings exhibit decreased cell viability in roots.

Fig 3

A) Root cell viability of Col-0 root tissues decreased as concentrations of DFPM increased. Wild-type (Col-0) seedlings were exposed to the indicated concentrations of DFPM or DMSO (control) for 24 hours and cell viability was visualized by staining with fluorescein diacetate (FDA) dye. B) Fluorescence intensity of FDA was measured in root tips exposed to different concentrations of DFPM from 0.5 to 10 μM. (n = 3–11 per condition. error bars represent s.e.m.) C) Roots of the DFPM-insensitive Ler accession [15] and victr-1 mutant (Col-0) seedlings exhibited a marginal decrease in cell viability staining after exposure to 3 μM DFPM for 24 hours. Constant gain and pinhole parameters were used for all images.