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. 2016 May 5;55(19):2735–2747. doi: 10.1021/acs.biochem.5b01285

Figure 4.

Figure 4

Investigation into the roles played by predicted catalytic residues for HexD. (A) Brønsted linear free energy plot of HexD-catalyzed hydrolysis of aryl GalNAc derivatives (1a and 1e–1j) as a function of aryl leaving group pKa: (●) wild-type HexD, (◆) D148A mutant, and (□) E149A mutant. (B) pH dependence of HexD hydrolysis of PNP-GalNAc (1a): (●) wild-type HexD (—) and (■) E149A mutant (---). Error bars represent the SEM. (C) Structures of aryl GalNAc substrates (1e–1j) and 4-methylumbelliferyl-GalNAc (3) used in Brønsted analyses.