. 2016 May 5;55(19):2735–2747. doi: 10.1021/acs.biochem.5b01285

Table 1. Kinetic Parameters^a for HexD Hydrolysis of Galactosaminides vs Glucosaminides.

substrate	K_M (mM)	k_cat (min^–1)	k_cat/K_M (min^–1 mM^–1)
PNP-GalNAc (1a)	0.0721 ± 0.0048	79.3 ± 1.2	1090 ± 75
PNP-GlcNAc (2a)	0.607 ± 0.053	30.4 ± 0.89	50.1 ± 4.6
3F4NP-GalNAc (1f)	0.172 ± 0.019	122 ± 3.9	707 ± 81
3F4NP-GlcNAc (2b)	0.852 ± 0.054	92.4 ± 2.7	109 ± 7.6

Calculated kinetic parameters represent the mean ± SEM of triplicate values.

Table 1. Kinetic Parametersa for HexD Hydrolysis of Galactosaminides vs Glucosaminides.