Table 1.

Relative enhancements of carbon resonances from phormidolide (1) biosynthetic feeding experiments.

Position	Chemical Shift (ppm)a	[1-13C] acetate	[2-13C] acetate	1Jcc values from[1, 213C2]acetate (Hz)	[CH3-13C] methionine
1	167.5	20.6	0.6	77.4	0.0
2	118.3	0.2	9	77.4	−0.1
3	152.1	32.8	1.3	52.1	0.5
4	134.1	1.0	9.2	52.1	−0.2
5	132.6	12.8	0.4	41.2	−0.3
6	44.1	0.6	11.8	41.4	0.1
7	73.1	16.9	0.6	38.3	−0.2
8	43.8	0.3	10.0	38.3	−0.1
9	141.5	15.4	0.2	54.4	−0.2
10	132.4	1.2	5.5	54.4	−0.4
11	133.4	17.5	0.7	41.4	−0.1
12	48.3	0.2	10.2	41.4	0.0
13	76.7
14	34.8	0.1	8.6	31.4	−0.2
15	79.6	19.7	0.6	39.1	−0.4
16	86.9	0.4	10.3	39.1	−0.1
17	69.7	15.7	0.4	49.8	−0.3
18	127	−0.3	8.6	49.8	−0.1
19	137.4	11.6	0.3	41.4	−0.4
20	42.3	0.2	11	41.4	0.0
21	77.5	9.1	0.4	37.4	−0.4
22	40.4	−0.4	5.2	38.3	−0.6
23	81.6	19.1	1.4	39.1	0.2
24	35.1	−0.3	7.4	39.4	−0.2
25	77.8	12.2	0.3	38.3	−0.4
26	41.5	0.0	9.7	38.3	−0.2
27	73.8	11.1	0.4	39.1	−0.2
28	39.2	−0.3	6.3	39.1	−0.3
29	70.5	8.6	0.0	39.1	−0.5
30	39.3	−0.4	4.3	38.3	−0.5
31	138.3	0.4	0.0		−0.5
32	158.4	−0.2	−0.1		−0.7
33	78.8	0.2	0.1		−0.5
34	13.9	0.5	9.0	39	−0.3
35	113.8	1.2	16.7	71.3	0.6
36	16.8	0.0	5.8	42.2	−0.4
37	21	1.7	0.8		10.9
38	17.3	0.2	9.9	43.8	0.1
39	13.7	0.8	0.8		8.8
40	21.6	0.6	0.2		6.5
41	5	0.2	0.0		5.3
42	122.1	−0.4	3.5		−0.5
43	55.6	0.0	0.0		4.7
44	173.7	8.6	0.2	57.5	−0.5

^aReferenced to δCDCl₃ at δ77.0. Bolded numbers indicate positions of ¹³C enrichment in phormidolide.