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. 2016 May 15;129(10):1989–2002. doi: 10.1242/jcs.180539

Fig. 3.

Fig. 3.

SNAIL1 protein level increases in breast tumors from mice with increased collagen deposition in the breast. (A–D) Picosirius Red staining of mammary gland from 13-week-old mice (A,C) and implanted PyMT breast tumor from WT (B) and Col1α1tmJae mice (D). (E) Western blot analysis of whole-tumor tissue lysates harvested from mammary tumors of FVB WT or FVB Col1α1tmJae mice transplanted with primary PyMT tumor cells. (F) Graph quantifying SNAIL1 protein level determined by densitometry and normalized to β-tubulin control. Results are presented as mean±s.d.; n=4. (G–L). Tumor epithelial K8 and SNAIL1 immunofluorescence of breast tumors from Col1α1tmJae mice (G–I) and WT mice (J–L). (M) A quantification of the percentage of K8+ tumor cells that were also SNAIL1+ in breast tumors from Col1α1tmJae mice and WT mice. Results are presented as mean±s.d. of five fields that were scored. *P<0.05 (unpaired, two-tailed Student's t-tests). Scale bars: 50 μm.