Fig. 6.

stam and hrs mutations cause abnormal localization of Rhomboid in endosomes. (A–E) Effects of stam and hrs mutations on Rhomboid (Rho) protein levels (A–Cʹ) and on Rhomboid–lacZ enhancer trap expression (D–E) are shown. Yellow arrows point to mutant clones, which are marked by the lack of GFP. (F–I″) Colocalization study of Rhomboid with YFP-tagged Rab5 (F–F″ and H–H″) or Rab7 (G–G″ and I–I″) in wild-type (WT) eye discs (F–G″) or eye discs with stam-mutant clones (H–I″). White arrowheads point to Rhomboid that did not show colocalization, and yellow arrowheads point to Rhomboid that showed colocalization with Rab5 or Rab7. (J–J‴) Colocalization study of Rhomboid and membrane Spi (mSpi) expressed in R8 cells (using the Sca–Gal4 driver; Sca-mSpi) in a stam-mutant clone (indicated by yellow arrowheads) and in adjacent wild-type tissues (indicated by white arrowheads). Multiple discs were examined, and representative results for each genotype are shown. Mutant clones in H–J‴ are marked by the lack of β-galactosidase staining (shown in blue). The complete genotypes of the flies analyzed are detailed in Table S1. Scale bars: 50 µm (A); 10 µm (F). The white dashed lines outline the stam-mutant clones.