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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Immunol Cell Biol. 2016 Jan 11;94(5):496–508. doi: 10.1038/icb.2016.5

Figure 8. CD8+ T cells from ΔznuA B. melitensis- or Rev-1-vaccinated and challenged CD4−/− mice show elevated IFN-γ production.

Figure 8

Splenic lymphocytes were isolated from the same C57BL/6, CD4−/−, and CD8−/− mice that were nasally vaccinated and subjected to pulmonary infection with wild-type B. melitensis strain 16M as described in Figure 5. Splenic CD4+ and CD8+ T cells were isolated (pooled from 2–3 mice/culture and at least three cultures/experiment) and co-cultured in the presence of mitomycin C-treated C57BL/6 Ag-presenting cells and 109 CFUs HKRB51 or media for 3 days. Harvested supernatants were evaluated for IFN-γ production by ELISA. Results are expressed as the mean ± SEM of triplicate cultures. Significant differences in IFN-γ production were determined: *P≤0.001, **P<0.05 (versus PBS-dosed mice); #P≤0.001 (differences between CD4+and CD8+ T cells to the same vaccine).