Table 1.

Serum cholesterol levels and blood pressures in Eln+/+ and Eln+/− mice crossed with Ldlr+/+ and Ldlr−/− mice and fed ND or WD for 16 weeks after weaning. Total cholesterol, triglyceride (tri), low density lipoprotein (LDL) and high density lipoprotein (HDL) serum concentrations were measured. Diet and Ldlr genotype significantly affect most measures of serum cholesterol, while Eln genotype does not. Systolic (sys) and pulse pressures measured with a solid-state catheter are significantly affected by diet, Ldlr genotype, and Eln genotype, while diastolic (dias) pressures are affected only by diet. Heart rate (hr) is not affected by any of the independent variables. Significance was determined by three-way ANOVA for diet, Ldlr genotype, Eln genotype and all two- and three-way interactions.

			serum cholesterol (mg/dl)				blood pressure (mmHg)
Diet	Ldlr	Eln	total	tri	LDL	HDL	sys	dias	pulse	hr (bpm)
ND	Ldlr+/+	Eln+/+	138 ± 32	62 ± 13	6 ± 3	69 ± 11	109 ± 12	74 ± 10	35 ± 4	497 ± 62
		Eln+/−	169 ± 39	55 ± 7	7 ± 3	83 ± 13	122 ± 19	79 ± 12	43 ± 11	514 ± 57
	Ldlr−/−	Eln+/+	441 ± 50	117 ± 35	123 ± 16	80 ± 14	114 ± 9	76 ± 7	37 ± 4	514 ± 68
		Eln+/−	432 ± 114	266 ± 181	170 ± 83	87 ± 13	122 ± 17	77 ± 11	44 ± 8	510 ± 82
WD	Ldlr+/+	Eln+/+	238 ± 77	66 ± 18	15 ± 5	120 ± 29	117 ± 12	78 ± 7	39 ± 6	484 ± 89
		Eln+/−	209 ± 77	58 ± 12	12 ± 3	114 ± 20	122 ± 12	81 ± 8	40 ± 7	506 ± 85
	Ldlr−/−	Eln+/+	1040 ± 143	740 ± 351	698 ± 229	99 ± 17	123 ± 16	82 ± 10	41 ± 8	482 ± 58
		Eln+/−	1001 ± 85	808 ± 215	596 ± 216	112 ± 10	141 ± 17	85 ± 9	56 ± 10	480 ± 58
P value		diet	<0.001	<0.001	<0.001	<0.001	0.002	0.011	0.006	0.154
		Ldlr	<0.001	<0.001	<0.001	0.632	0.014	0.299	0.001	0.786
		Eln	0.596	0.215	0.628	0.122	<0.001	0.115	<0.001	0.565
	interactions		diet × Ldlr (<0.001)	diet × Ldlr (<0.001)	diet × Ldlr (<0.001)	-	-	-	diet × Ldlr (0.027), diet × Ldlr × Eln (0.018)	-
N/group			5 – 7	5 – 7	5 – 7	5 – 7	9 – 17	9 – 17	9 – 17	9 – 17

Italics indicate P < 0.05. Data are presented as mean ± SD.