Table 2.

Levels of inflammatory cytokines and TGF-β1 were measured using electrochemiluminescence immunoassays on serum from Eln+/+Ldlr−/− and Eln+/−Ldlr−/− mice and fed ND or WD for 16 weeks after weaning. IL6, IL10, CXCL1, and TNF levels are significantly affected by diet, but not Eln genotype. TGF-β1 levels are not affected by diet or Eln genotype. Significance was determined by two-way ANOVA for diet, Eln genotype and the interaction between diet and Eln genotype.

			inflammatory cytokines (pg/ml)				TGF-β1 (ng/ml)
Diet	Ldlr	Eln	IL6	IL10	CXCL1	TNF	TGF-β1
ND	Ldlr−/−	Eln+/+	12 ± 5	10 ± 2	29 ± 10	8 ± 2	39 ± 10
		Eln+/−	13 ± 3	10 ± 3	26 ± 8	7 ± 1	52 ± 12
WD	Ldlr−/−	Eln+/+	89 ± 28	29 ± 20	185 ± 160	23 ± 7	28 ± 30
		Eln+/−	86 ± 73	20 ± 6	202 ± 144	29 ± 15	37 ± 26
P value		Diet	0.021	0.005	0.019	0.007	0.332
		Eln	0.964	0.231	0.913	0.697	0.412
	Diet × Eln		0.956	0.274	0.873	0.579	0.879
N/group			2 – 6	2 – 6	2 – 6	2 – 6	2 – 6

Italics indicate P < 0.05. Data are presented as mean ± SD.