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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Atherosclerosis. 2016 Mar 26;249:1–9. doi: 10.1016/j.atherosclerosis.2016.03.032

Fig. 3. Histological assessment of mild and severe CAD and collagen deposition.

Fig. 3

(A–C) Verhoeff-Van Giesen staining of elastin in a coronary arterial ring in lean (A), mild CAD (IVUS-detected plaque burden < 30%) (B), and severe CAD (IVUS-detected plaque burden > 30%) (C). L = Lumen; M = Media (Wall). I = Intima (plaque). Black arrows indicate the internal elastic lamina adjacent to early atherosclerotic lesion. (D – F) Picrosirius red staining of collagen deposition in lean (D), mild CAD (E), and severe CAD (F) coronary arterial rings. (G) % plaque burden is increased with severe CAD. (H) Intima/media ratio is increased with severe CAD. (I) Intimal collagen deposition is increased in severe CAD. (J) Medial collagen deposition demonstrates a trend (p=0.08) toward increase in severe CAD. (Mild CAD = 9 pigs; Severe CAD = 5 pigs)*p <0.05.