Fig. 3. Sequence analysis of selected clones.
Specific CDR sequences were enriched during selection. The sequences identified with the highest frequency are labeled sequence A, B, and C. All other sequences were found at a frequency of less than 10%. (A) Oligonucleotide distribution of CDRs found against the EZH2 target after three rounds of biopanning shows a strong consensus in each CDR (represented as sequence A). (B) Oligonucleotide distribution after two rounds of biopanning against the EZH2 target. The same sequences A, B, and C found in round 3 were also identified in round 2. (C) The enriched sequences A, B, and C found after two and three rounds of panning against EZH2 protein fragment.