Figure 1.

Identification of different DC subsets in tonsils. Representative gating strategy to define the main tonsillar DC subsets. (A) Gating strategy to identify viable Lin^neg HLA-DR⁺ tonsillar mononuclear cells. (B,C) The panels represent tonsil mononuclear cells gated as Lin^neg and analyzed for HLA-DR, CD11c, and CD304. (D,E) The panels show gated Lin^neg HLA-DR⁺ CD11c⁺ cells (red arrows) analyzed for expression of CD141, clec9A, CD1c, and CD14. The main tonsillar subsets are indicated. (F) Plot represents gated Lin^neg cells analyzed for expression of HLA-DR and CD207. These data are representative of 12 independent experiments (10 children and 2 adults).