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. 2016 May 25;7:187. doi: 10.3389/fphys.2016.00187

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Copyright © 2016 Moo, Fortuna, Sibole, Abusara and Herzog.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Local dispersion of sarcomere lengths at full dorsiflexion, intermediate ankle angle, and full plantarflexion given by (A) the local standard deviation (SD) and, (B) the local coefficient of variation (CV). For each TA site of 159 × 159 μm, the local dispersion of sarcomere lengths was measured from four representative image bands that were of 4.7 μm wide and contained 15–20 sarcomeres in series. The graphs show the average values of the local SDs (a) and local CVs (b) for the five mice. Sarcomeres at the “proximal” TA site had higher local SD and local CV compared to sarcomeres at the remaining TA sites. ^*Indicates significant differences in local SD and local CV compared to the sarcomeres at the proximal TA site and a given ankle joint angle (p < 0.05). ^aIndicates significant differences in local SD and local CV compared to sarcomeres in the fully dorsi-flexed position (p < 0.05; see Section Statistical analysis in the “Methods” for more details).