Fig. 1.

Increased fatty streak formation in aortic wall of female LI-IGF-I^−/− mice. Male and female LI-IGF-I^−/− and control mice (n = 9–16/group) received an atherogenic (modified Paigen) diet from 6 months of age. At the end of the study (12 months of age), a fatty streak area and b lipid deposition were quantified in Oil Red O-stained aortic root cryosections. c, d Fatty streak area and lipid deposition are given as % of aortic wall area. One section per mouse was analyzed at the level of the aortic cusps. e, f Representative photomicrographs from female e control and f LI-IGF-I^−/− mice are shown. Values in a–d are given as means (SEM). The analyses of fatty streaks and lipid deposition were performed in all mice. p values were calculated using unpaired t tests. In e and f, arrows point at fatty streaks