Figure 11.

Neogenin regulation of BMP2 activation of YAP via RhoA. A, Western blot analysis of RhoA activity (GTP bound RhoA) in WT and neogenin mutant astrocytes in response to BMP2 (100 ng/ml) treatment at indicated time. B, Quantitative analysis of the relative RhoA activity in (A) (n = 3 per group, normalized to 0 min). C, D, Triple immunostaining of YAP (green), T13N-RhoA-myc (red), and F-actin (blue) in WT astrocyte (C), and YAP (green), Q63L-RhoA-myc (red), and F-actin (blue) in neogenin mutant astrocyte (D) before and after BMP2 treatment. E, F, Quantitative analysis of nuclear YAP intensity in WT astrocytes transfected with T13N-RhoA-myc (E) (n = 13 per group), or in neogenin mutant astrocytes transfected with Q63L-RhoA-myc (F) (n = 9 per group). Scale bars, 20 μm. Data are mean ± SEM. **p < 0.01, compared with control group (Student's t test).