Generation of neonestin-CKO, neoGFAP-CKO and neoNex-CKO mice. A, Diagram of how to generate neonestin-CKO mice. B, Genotyping of neonestin-CKO mice. C, Western blot analysis of neogenin expression in homogenates of different brain regions of P7 neof/f and neonestin-CKO mice. D, Western blot analysis of neogenin expression in homogenates of different brain regions of P3 neof/f and neoGFAP-CKO mice. E, Western blot analysis of neogenin expression in homogenates of different brain regions of P10 neof/f and neoNex-CKO mice. Hipo, Hippocampus.