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. 2016 May 25;36(21):5833–5849. doi: 10.1523/JNEUROSCI.4487-15.2016

Figure 9.

Figure 9.

Requirement of neogenin for YAP activation and interaction with p-Smad1/5/8 in neocortical astrocytes in response to BMP2 treatment. A, Western blot analysis showed BMP2 signaling proteins in WT and neogeninm/m astrocytes before and after BMP2 treatment. B–E, Quantitative analysis of the pSmad1/5/8 (B), Smad1 (C), pYAP (D), and YAP (E) from data in A. F, Western blot analysis detected BMP2-downstream signaling proteins in the cytoplasm and nuclear fractions of astrocytes with and without BMP2 treatment. G, H, Quantitative analysis of the relative Smad1 (G) and YAP (H) level in F (n = 3 per group). I, Double immunostaining of YAP (green) and p-Smad1/5/8 (red) in astrocytes after BMP2 treatment. J, Double immunostaining of YAP (green) and p-Smad1/5/8 (red) in NSCs after BMP2 treatment. K, Western blot analysis showed nuclear protein coimmunoprecipitation results by nuclear complex coimmunoprecipitation assays in WT and neogenin mutant astrocytes before and after BMP2 treatment (100 ng/ml). Scale bars, 20 μm. Data are mean ± SEM. **p < 0.01, compared with control group (Student's t test).