FIG 1.
OAg-AcrA recombinant glycoprotein as a novel antigen for the diagnosis of porcine brucellosis. Six animals were intramuscularly inoculated with 5 × 106 CFU of B. suis 1330, and serum samples were obtained before the infection and 16 and 60 days postinfection. (A) Glyco-iELISA analysis of the samples. The reactivity values are relative to the positive control included in each assay run. (B) Western blot analysis of the serum samples obtained before infection (Pre) and 60 days postinfection (Post). For animal 1, the analyzed sample corresponded to that obtained at 16 days postinfection. NG, nonglycosylated AcrA; G, glycosylated AcrA (OAg-AcrA). The positions of molecular mass standards (in kDa) are indicated on the left. The arrows on the right indicate the migration positions of nonglycosylated AcrA.