TABLE 1.
Key assay characteristicsa
| Assay | Reagent manufacturer (location) | Chemistry | Instrumentation (manufacturer) | Total reaction mixture vol (μl) | Sample vol (μl) |
|---|---|---|---|---|---|
| ELITech | ELITech Group (Bothell, WA) | TaqMan, MGB Alert probe/primer mixture | 7500 real-time PCR instrument (Life Technologies, Grand Rapids, NY) | 25 | 5 |
| Focus | Focus Diagnostics (Cypress, CA), | Scorpion-labeled primers | 3 M integrated cycler (Focus Diagnostics) | 10 | 5 |
| Luminex | Luminex Corporation (Austin, TX) | MultiCode-labeled primers | 7500 real-time PCR instrument | 25 | 5 |
| LDT | In-house developed | TaqMan | 7500 real-time PCR instrument | 25 | 5 |
a
See reference 20.