FIG 1.

Nucleotide sequences of the in-run controls used for the 5′ nuclease genotyping assay. The nucleotide sequences of wild-type Ultramer oligonucleotide (Wt) and Ultramer oligonucleotides containing the two most frequent macrolide resistance mutations, A2058G and A2059G, are shown. The location of the forward primer is indicated in blue and that of the reverse primer is indicated in green. Arrows indicate the 5′ to 3′ direction of the primer sequences. The nucleotide sequences of the three probes, wild-type probe, A2058G probe, and A2059G probe, are shown in the red boxes.