TABLE 3.
Diagnostic accuracy of the best-performing cocktail ELISA compared to the OIE-prescribed serological tests for CBPP diagnosisf
| ELISA components or test | No. diagnosed positive/total no. positive | No. diagnosed negative/total no. negative | Sensitivitya (95% CI) | Specificityb (95% CI) | PPVc (95% CI) | NPVd (95% CI) | Overall diagnostic accuracye (95% CI) | % agreement (95% CI) |
|---|---|---|---|---|---|---|---|---|
| MSC_0136/MSC_0636 | 107/125 | 27/28 | 85.6 (78.0–91.0) | 96.4 (79.8–99.8) | 99.1 (94.2–99.9) | 60.0 (44.4–73.9) | 87.6 (82.4–92.8) | NA |
| CFT | 101/114 | 28/28 | 88.5 (80.9–93.5) | 100 (85.0–100) | 100 (95.4–100) | 68.3 (51.8–81.4) | 90.8 (86.1–95.6) | NA |
| cELISA | 103/125 | 27/27 | 82.4 (74.3–88.4) | 100 (84.5–100) | 100 (95.5–100) | 55.1 (40.3–69.1) | 85.5 (79.9–91.1) | NA |
| Comparison of MSC_0136/0636 with CFT | 91 | 35 | NA | NA | NA | NA | NA | 88.7 (83.5–93.9), κ = 0.73 |
| Comparison of MSC_0136/0636 with cELISA | 101 | 42 | NA | NA | NA | NA | NA | 94.1 (90.3–97.8), κ = 0.86 |
Diagnostic sensitivity: TP/(TP + FN) × 100%, where TP is the number of true-positive samples and FN is the number of false-negative samples.
Diagnostic specificity: TN/(TN + FP) × 100%, where TN is the number of true-negative samples and FP is the number of false-positive samples.
PPV, positive predictive value: TP/(TP + FP) × 100%.
NPV, negative predictive value: TN/(TN + FN) × 100%.
Overall diagnostic accuracy: (TP + TN)/(TP + TN + FP + FN) × 100%.
This table represents results obtained through the use of panel B and C serum samples only. κ, Cohen's kappa index value, classified as very good (1 to 0.76), good (0.75 to 0.61), acceptable (0.6 to 0.4), and poor (<0.4) (31); NA, not applicable; CI, confidence interval.