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. 2016 May 25;7:185. doi: 10.3389/fphys.2016.00185

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Copyright © 2016 Coisne, Hallier-Vanuxeem, Boucau, Hachani, Tilloy, Bricout, Monflier, Wils, Serpelloni, Parissaux, Fenart and Gosselet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the expressions of ABCA1 and SR-BI upon CD-treatment in SMCs. Twenty-four hours after treatment (CDs 1 mM, solid bars), mRNA expressions of ABCA1 (A) and SCARB1 (C) were assessed and compared with control (DMEM treatment, open bar). Each bar represents the mRNA expression normalized against the housekeeping gene β-ACTIN ± SEM (n = 6). Control condition (without CD treatment) is used as the reference level and set to 1. A one-way ANOVA test followed by Dunnett's test for multiple comparisons was applied with ^***p < 0.001 compared with control. Normalized protein expression levels for ABCA1 (B) and SR-BI (D) were assessed by immunoblotting. β-ACTIN was used as the loading control. Results correspond to the optical density measured in three experiments. Control condition (without any CD treatment) is used as the reference level and set to 1.