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. 2016 May 25;7:185. doi: 10.3389/fphys.2016.00185

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Copyright © 2016 Coisne, Hallier-Vanuxeem, Boucau, Hachani, Tilloy, Bricout, Monflier, Wils, Serpelloni, Parissaux, Fenart and Gosselet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of 10 μM T0901317 on ABCA1, ABCG1, and SR-BI expression in ABAE (A) and SMCs (B) treated or not with Rameβ. Cells were first labeled as previously described and then treated with 1 mM of Rameβ supplemented or not with 10 μM T0901317. Transcriptional and protein expression of ABCA1, ABCG1, and SR-BI were studied by real-time PCR and immunoblot in ABAE (A) and SMCs (B) and compared with control (DMSO treatment, open bar). For mRNA study, each bar represents the mRNA expression normalized against the housekeeping gene β-ACTIN ± SEM (n = 6). For protein expression study, β-ACTIN was used as the loading control and results correspond to the optical density measured in two independent experiments performed in triplicate. Control condition is used as the reference level and set to 1. A one-way ANOVA test followed by Dunnett's test for multiple comparisons was applied with ^**p < 0.01; ^***p < 0.001 compared with control condition.