FIGURE 6.

PCR expression analysis of selected differentially expressed buffalograss genes. 17, 50, 95, and P refer to NE BG 7-3459-17, NE BG 7-3453-50, 95–55 and Prestige, respectively. C and T followed by the buffalograss lines indicate untreated and treated samples. Ubiquitin conjugating enzyme (UCE) is the endogenous gene and positive control. A, M, O, P, and Q are genes which had >10 read counts for both treated and untreated resistant buffalograss lines (95T, 17T, 95C, and 95C) and zero read counts for all susceptible lines (PT, 50T, PC, and 50T). The read counts of treated resistant lines were up-regulated in C, E, and F compared to the treated susceptible lines. J and L genes had higher read counts in untreated resistant lines compared to the untreated susceptible lines. LAD is the molecular marker.