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. 2016 May 25;7:720. doi: 10.3389/fpls.2016.00720

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Copyright © 2016 Wang, Sun, Jia, Wang, Gong and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yeast two-hybrid assays of full-length MdATG8i and MdATG7s proteins. For testing protein–protein interactions, transformed yeast cells were grown on selective SD medium lacking Trp and Leu (Left) or SD medium lacking Trp, Leu, Ade, and His nutrients (Right). Empty vectors pGBKT7 and pGADT7 and their combinations with MdATG8i and MdATG7s were used as negative controls. Combinations: 1, MdATG8i BK + MdATG7a AD; 2, MdATG8i BK + MdATG7b AD; 3, MdATG8i BK + AD; 4, BK + MdATG7a AD; 5, BK + MdATG7b AD; and 6, BK + AD.