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. 2016 May 25;7:720. doi: 10.3389/fpls.2016.00720

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Copyright © 2016 Wang, Sun, Jia, Wang, Gong and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heterologous expression of MdATG8i in Arabidopsis and growth phenotype. (A) PCR with gDNA; (B) PCR with cDNA; Lanes M, molecular marker DL2000; V, positive vector containing pGWB406-MdATG8i plasmid; WT, non-transformed wild-type; OE-6 and -7, MdATG8i-transgenic lines; (C) qRT-PCR analysis of MdATG8i transcripts in Arabidopsis lines OE-6 and OE-7; (D) Growth phenotype comparison of WT and transgenic lines under LD photoperiod; (E) Growth for rosettes from WT or transgenic lines under SD photoperiod; Data are means ± SD of 20 replicates. *, ** or *** indicates the statistically significant differences determined by Student's t-tests at P < 0.05, P < 0.01, or P < 0.001, respectively.