Skip to main content
. 2016 May 25;7:720. doi: 10.3389/fpls.2016.00720

Table 1.

Primers used in this study.

Primer Purpose/Vector Sequence (5′–3′)
MdATG8i–F Clone/pMD19T–simple F: ATGGGGAAGATCCAATCTTTCAAG
MdATG8i–R R: TTAGCCAAAGGTTTTCTCGCTGCT
BK–MdATG8iEcoR I –F Yeast two–hybrid/pGBKT7 F: CCGGAATTCATGGGGAAGATCCAATC
BK–MdATG8iBamH I –R R: CGCGGATCCTTAGCCAAAGGTTTTC
AD–MdATG7aEcoR I –F Yeast two–hybrid/pGADT7 F: CCGGAATTCATGGAGGGAGGC
AD–MdATG7aCla I –R R: CCATCGATTCAAACTTCAACCAAATCATC
AD–MdATG7bEcoR I –F F: CCGGAATTCATGGAGCGAGGC
AD–MdATG7bCla I –R R: CCATCGATTCAAACCTCAACCAAATCATC
pro–MdATG8i–F Promoter clone/pMD19T–simple F: GAACAACAAACAATGATTATTTGACA
pro–MdATG8i–R R: GATTGGATCTTCCCCATAAAGC
OE–MdATG8i–F Plant overexpression and Subcellular localization /pGWB406–GFP F: AAAAAAGCAGGCTTCATGGGGAAGATCCAATC
OE–MdATG8i–R R: CAAGAAAGCTGGGTTTTAGCCAAAGGTTTTCTC
q MdATG8i–F qRT–PCR F: GCAGCAGGCTTCACTTGACTCC
q MdATG8i–R R: GGAATCCATGCGACTGGCTGTT
AtUBQ10–F F: TTCACTTGGTCCTGCGTCTTC
AtUBQ10–R R: CATCAGGGATTATACAAGGCCCC
attB1 Adapter primer for gateway vector F: GGGGACAAGTTTGTACAAAAAAGCAGGCT
attB2 R: GGGGACCACTTTGTACAAGAAAGCTGGGT

Restriction sites and adapters are underlined.