Table 1.
Primer | Purpose/Vector | Sequence (5′–3′) |
---|---|---|
MdATG8i–F | Clone/pMD19T–simple | F: ATGGGGAAGATCCAATCTTTCAAG |
MdATG8i–R | R: TTAGCCAAAGGTTTTCTCGCTGCT | |
BK–MdATG8i–EcoR I –F | Yeast two–hybrid/pGBKT7 | F: CCGGAATTCATGGGGAAGATCCAATC |
BK–MdATG8i–BamH I –R | R: CGCGGATCCTTAGCCAAAGGTTTTC | |
AD–MdATG7a–EcoR I –F | Yeast two–hybrid/pGADT7 | F: CCGGAATTCATGGAGGGAGGC |
AD–MdATG7a–Cla I –R | R: CCATCGATTCAAACTTCAACCAAATCATC | |
AD–MdATG7b–EcoR I –F | F: CCGGAATTCATGGAGCGAGGC | |
AD–MdATG7b–Cla I –R | R: CCATCGATTCAAACCTCAACCAAATCATC | |
pro–MdATG8i–F | Promoter clone/pMD19T–simple | F: GAACAACAAACAATGATTATTTGACA |
pro–MdATG8i–R | R: GATTGGATCTTCCCCATAAAGC | |
OE–MdATG8i–F | Plant overexpression and Subcellular localization /pGWB406–GFP | F: AAAAAAGCAGGCTTCATGGGGAAGATCCAATC |
OE–MdATG8i–R | R: CAAGAAAGCTGGGTTTTAGCCAAAGGTTTTCTC | |
q MdATG8i–F | qRT–PCR | F: GCAGCAGGCTTCACTTGACTCC |
q MdATG8i–R | R: GGAATCCATGCGACTGGCTGTT | |
AtUBQ10–F | F: TTCACTTGGTCCTGCGTCTTC | |
AtUBQ10–R | R: CATCAGGGATTATACAAGGCCCC | |
attB1 | Adapter primer for gateway vector | F: GGGGACAAGTTTGTACAAAAAAGCAGGCT |
attB2 | R: GGGGACCACTTTGTACAAGAAAGCTGGGT |
Restriction sites and adapters are underlined.