Intrapatient HIV-1 genetic-diversity analysis was performed by quantifying the unique deep-sequencing reads with a frequency of ≥10, corresponding to 105-bp fragments from the PR-, RT-, and INT-coding regions for each patient. Groups I, II, III, and IV were defined as shown in Table 1. Means ± standard deviations and statistically significant differences (unpaired t test; P values) are indicated.