FIG 2.

Time-dependent accumulation and efflux of Sb^III in Leishmania lines. (a) L. major lines (1 × 10⁸ promastigotes/ml) carrying pUCNEO (control) and overexpressing LABCG2 were incubated with 100 μM Sb^III, and samples were taken after different time points. Antimony accumulation was measured by ICP-MS. (b) An efflux assay was performed after incubation of Leishmania lines with compensated concentrations of Sb^III for 1 h to ensure similar labeling in the different lines. The parasites were then washed and resuspended in PBS without Sb^III and pelleted at different time points. The data are the means ± standard deviations of results from three independent experiments performed in duplicate. Significant differences versus the control line were determined by using the Student t test (*, P < 0.01).