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Determination of thiol levels in Leishmania lines. Promastigotes (107/ml) of L. major lines carrying pUCNEO (control) and overexpressing LABCG2 were incubated for 15 min at 37°C with 2 μM CellTracker and quantified by flow cytometry. The data are the means ± standard deviations of results from three independent experiments. Significant differences versus the control line were determined by using the Student t test (*, P < 0.01).
