TABLE 1.
Drug resistance profile in promastigote L. major linesa
| Drug | Mean EC50 (μM) ± SD (RI)b |
||
|---|---|---|---|
| pUCNEO | LABCG2 | LABCG2rev 90D | |
| SbIII | 16.02 ± 2.63 | 118.84 ± 11.50 (7.4)* | 11.53 ± 0.69 (0.7) |
| AsIII | 0.99 ± 0.30 | 6.02 ± 1.50 (6.0)* | 1.32 ± 0.02 (1.3) |
| Amphotericin B | 2.27 ± 0.73 | 2.33 ± 0.45 (1.0) | — |
| Miltefosine | 18.25 ± 0.22 | 17.64 ± 1.70 (0.9) | — |
| Pentamidine | 0.66 ± 0.11 | 1.03 ± 0.11 (1.6)* | 0.83 ± 0.03 (1.3) |
| Tafenoquine | 12.87 ± 3.16 | 15.92 ± 7.50 (1.2) | — |
| Sitamaquine | 21.88 ± 5.43 | 18.02 ± 0.82 (0.8) | — |
| Primaquine | 5.43 ± 0.32 | 6.11 ± 0.05 (1.1) | — |
| Chloroquine | 10.99 ± 0.53 | 9.37 ± 1.67 (0.8) | — |
| Daunomycin | 0.56 ± 0.09 | 1.06 ± 0.16 (1.9)* | 0.67 ± 0.06 (1.2) |
| Mefloquine | 2.02 ± 0.19 | 3.04 ± 0.34 (1.5) | — |
| Quinine | 23.62 ± 2.84 | 29.69 ± 0.26 (1.3) | — |
| Perifosine | 20.99 ± 1.95 | 20.96 ± 1.27 (1.0) | — |
| Vinblastine | 10.31 ± 2.16 | 13.25 ± 2.65 (1.3) | — |
a
Promastigotes of Leishmania lines were grown for 72 h at 28°C in the presence of increasing concentrations of drugs. Cell viability was determined by using an MTT-based assay as described in Materials and Methods. Bold font represents significant resistance.
b
Resistance indexes (RI) were calculated by dividing the EC50 for the Leishmania line overexpressing LABCG2 and LABCG2rev 90D by that for the Leishmania control line (pUCNEO). Data are the means ± standard deviations of results from three independent experiments. Significant differences were determined by using the Student t test (*, P < 0.01). —, not determined.