TABLE 2.
Heavy metal resistance profiles of L. major promastigote linesa
| Metal | Mean EC50 (μM) ± SD (RI)b |
|||
|---|---|---|---|---|
| pUCNEO | LABCG2 | 2-LABCG2 | GFP-LABCG2 | |
| SbIII | 16.02 ± 2.63 | 118.84 ± 11.50 (7.4)* | 42.92 ± 7.75 (2.7)* | 34.93 ± 1.00 (2.2)* |
| AsIII | 0.99 ± 0.30 | 6.02 ± 1.50 (6.0)* | 2.72 ± 0.78 (2.7)* | 3.20 ± 0.18 (3.2)* |
| CdII | 58.77 ± 0.82 | 52.79 ± 4.21 (0.9) | — | — |
| CoII | 28.46 ± 4.05 | 36.57 ± 5.27 (1.3) | — | — |
| CuII | 43.52 ± 7.02 | 60.68 ± 4.99 (1.4) | — | — |
a
Promastigotes of Leishmania lines were grown for 72 h at 28°C in the presence of increasing concentrations of metals. Cell viability was determined by using an MTT-based assay as described in Materials and Methods. Bold font represents significant resistance.
b
Resistance indexes (RI) were calculated by dividing the EC50 for Leishmania line overexpressing LABCG2, LABCG2rev at 90 days (LABCG2rev 90D), second-event LABCG2 (2-LABCG2), and GFP-LABCG2 by that for Leishmania control line (pUCNEO). Data are the means ± standard deviations of results from three independent experiments. Significant differences were determined by using the Student t test (*, P < 0.01). —, not determined.