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Effect of C. difficile VPI 10463 supernatant on the relative mRNA expression of Caco-2 cells in the presence or absence of B. clausii O/C supernatant. Cd-SN and OC-SN were preincubated (Cd-SN/OC-SN), with or without protease inhibitor (PMSF), before contact with cells. Bars represent the relative expression of genes (in the three independent assays performed) encoding RhoB (A), ZO-1 (B), and ROCK2 (C). **, significant differences from the corresponding control (P < 0.01).
