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Purification of the 27-kDa protease from B. clausii culture supernatant
| Step | Total amt of proteins (mg) | Total acta (U) | Sp act (U · mg of protein−1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Culture supernatant | 3.80 | 37.02 | 9.74 | 100 | |
| Ultrafiltration concentrate | 2.85 | 38.20 | 13.40 | 103 | 1.37 |
| Superdex 75 chromatography | 0.175 | 22.67 | 129.54 | 61.2 | 13.30 |
One unit of activity was defined as the amount of enzyme that released 1 μmol of pNA per min at 37°C and pH 8.
