TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| S. aureus strains | ||
| RN4220 | 8325-4, r−, initial recipient for modification of plasmids which are introduced into S. aureus from E. coli | NARSAb |
| N315 | HA-MRSA, SCCmec type II | NARSA |
| N315ΔspoVG | N315 strain deletion of spoVG | This study |
| E. coli strains | ||
| Trans1-T1 | Clone host strain; F− Φ80 (lacZ) ΔM15ΔlacX74 hsdR (rK− mK+) ΔrecA1398 endA1 tonA | TransGen |
| BL21(DE3) | Express strain; F− ompT hsdSB (rB− mB−) gal dcm (DE3) | TransGen |
| Plasmids | ||
| pBTs | Shuttle vector, temp sensitive, Ampr Chlr | |
| pBTsΔspoVG | pBTs derivative, for spoVG deletion in strain N315, Ampr Chlr | This study |
| pET28a(+) | Expression vector with a hexahistidine tag, Kanr | Novagen |
| pETSpoVG | pET28a(+) derivative, with ORF of spoVG, Kanr | This study |
| pLI50 | Shuttle vector, Ampr Chlr | Addgene |
| pLIspoVG | pLI50 derivative, harboring ORF of spoVG and its promoter, Ampr Chlr | This study |
a
r−, restriction system negative; Kanr, kanamycin resistant; Ampr, ampicillin resistant; Chlr, chloramphenicol resistant.
b
NARSA, Network on Antimicrobial Resistance in Staphylococcus aureus (https://www.beiresources.org/Collection/53/NARSA.aspx).