TABLE 1.
Phenotypes and topoisomerase and PMQR genotypes of 21 human ciprofloxacin- and cefotaxime-resistant S. enterica serovar Indiana isolates
| Strain | Source | Age | Yr of isolation | MIC (mg/liter) |
QRDR amino acid substitutionsa |
Gene(s) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CIP | CTX | gyrA | gyrB | parC | parE | PMQR | β-Lactamase | ||||
| P1 | Zhoukou | 1 mo | 2009 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-15 |
| P2 | Jiyuan | 7 yr | 2010 | 8 | 128 | S83F/D87G | WT | T57S/S80R | WT | oqxAB | blaCTX-M-14 |
| P3 | Zhoukou | 28 yr | 2010 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P4 | Zhenzhou | 1 mo | 2011 | 32 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr | blaCTX-M-15 |
| P5 | Jiyuan | 28 yr | 2011 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P6 | Jiyuan | 1 mo | 2011 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P7 | Jiyuan | 36 yr | 2011 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P8 | Jiyuan | 1 yr | 2011 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P9 | Shangqiu | 22 yr | 2011 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-15 |
| P10 | Shangqiu | 50 yr | 2011 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-15 |
| P11 | Shangqiu | 49 yr | 2011 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-14, blaCTX-M-15 |
| P13 | Shangqiu | 1 mo | 2011 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-27 |
| P14 | Zhenzhou | 4 mo | 2012 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P17 | Shangqiu | 15 mo | 2012 | 32 | 128 | S83F/D87G | WT | T57S/S80R | WT | oqxAB | blaCTX-M-14 |
| P18 | Zhoukou | 17 mo | 2012 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-15 |
| P19 | Zhoukou | 9 mo | 2012 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P21 | Shangqiu | 5 mo | 2012 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P22 | Shangqiu | 74 yr | 2012 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P23 | Dengfeng | 6 mo | 2012 | 128 | 128 | S83F/D87G | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-65 |
| P24 | Zhenzhou | 9 yr | 2013 | 128 | 128 | S83F/D87N | WT | T57S/S80R | WT | aac(6′)-Ib-cr, oqxAB | blaCTX-M-14 |
| P26 | Zhoukou | 9 mo | 2013 | 32 | 128 | S83F/D87G | WT | T57S/S80R | WT | oqxAB | blaCTX-M-14 |
a
WT, wild type.