TABLE 3.
Synthetic oligonucleotide primers
| Plasmid constructed and primer designation | Sequencea (5′-3′) | Geneb |
|---|---|---|
| pKOR1-graR(N197S) | ||
| attB1-graR-FW | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTATTGAAGATTTCGGCAAAGTAATGGATACA | sav0659 |
| attB2-graR-RV | GGGGACCACTTTGTACAAGAAAGCTGGGTATAATCAACTGTATGACGTT | sav0659 |
| pKOR1-msrR(E146K) | ||
| attB1-msrR-FW | GGGGACAAGTTTGTACAAAAAAGCAGGCTACAAGGTGGACAATCAAGAACAGATTCTATCATGGTTGTTC | sav1362 |
| attB2-msrR-RV | GGGGACCACTTTGTACAAGAAAGCTGGGTTCGTCAATGTAAAATTATTAGAAGGTCGTTCGGATGAACAAT | sav1362 |
| pKOR1-Δsle1 | ||
| attB1-Δsle1-FW | GGGGACAAGTTTGTACAAAAAAGCAGGCTAGTAATTGCAGCTATTATTGGGACAAGCGCGATTAGCGCTGTTGCGGCA | sav0465 |
| attB2-Δsle1-RV | GGGGACCACTTTGTACAAGAAAGCTGGGTAGTTTAGGATTCAATCCAACTTTTCAGCTTGTGAAATGTA | sav0465 |
a
Underlining indicates att sequences introduced into the primer.
b
The genes listed are specific for the chromosome of strain Mu50 (GenBank accession no. AP003367).