FIG 1.

Screening for novel base formulations. Shown is the amount of P. aeruginosa biofilm remaining after treatment with enzyme-free base formulations (light gray) compared to that remaining after treatment with the same base formulations containing a mixture of seven enzymes (dark gray). The y axis represents the biofilm amount quantified by crystal violet staining relative to the negative control (biofilm treated with WSH containing no detergents or enzymes). Error bars represent data from 6 individual replicates. In a first round of screening, 4 base formulations were selected (a) and further optimized in a second round of screening (b). B3A is a derivative of B2A, B3B is a derivative of B2B, B3D1/2 is a derivative of B2D, and B3I1/2/3 is a derivative of B2I.