TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference |
|---|---|---|
| E. coli strains | ||
| DH5α | ϕ80d lacZΔM15 endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 F−Δ(lacZYA-argF) U169 | 84 |
| S17-1 | thi pro hsdR recA Tra+ | 85 |
| P. aeruginosa strains | ||
| K767 | PAO1 prototroph | 86 |
| K3159 | K767 ΔamgR | 60 |
| K1525 | K767 ΔmexXY | 87 |
| K3162 | K767 ΔPA2798 | 60 |
| K3696 | K767 rpoBD521Y (Rifr) | This study |
| K3249 | K767 amgSR182C | 62 |
| K3260 | K767 amgSV121G | 62 |
| K3584 | K3260 ΔamgR | 62 |
| K3585 | K3249 ΔamgR | 62 |
| K3589 | K767 ΔyccA | 61 |
| K3590 | K767 ΔhtpX | 61 |
| K3591 | K767 ΔPA5528 | 61 |
| K3593 | K767 ΔyccA ΔhtpX | 61 |
| K3594 | K767 ΔhtpX ΔPA5528 | 61 |
| K3595 | K767 ΔPA5528 ΔyccA | 61 |
| K3596 | K767 ΔhtpX ΔPA5528 ΔyccA | 61 |
| K2154 | Aminoglycoside-resistant CF isolate | 68 |
| K2156 | Aminoglycoside-resistant CF isolate | 68 |
| K2157 | Aminoglycoside-resistant CF isolate | 68 |
| K2158 | Aminoglycoside-resistant CF isolate | 68 |
| K2162 | Aminoglycoside-resistant CF isolate | 68 |
| K3630 | K2156 ΔamgR | This study |
| K2167 | K2156 ΔmexXY | 68 |
| Plasmids | ||
| pEX18Tc | Broad-host-range gene replacement vector; sacB Tcr | 88 |
| pCG005 | pEX18Tc::ΔamgR | 60 |
a
RIFr, rifampin resistant; Tcr, tetracycline resistant.